The Influence of Anesthesia on Dopamine Signaling in the Rat Dorsal Striatum
Faculty Mentor
David Daberkow
Presentation Type
Poster
Start Date
5-8-2024 11:15 AM
End Date
5-8-2024 1:00 PM
Location
PUB NCR
Primary Discipline of Presentation
Biology
Abstract
The striatum is a brain structure involved in reward learning and motor control. It receives input from the neurotransmitter dopamine (DA). Fast-scan cyclic voltammetry (FSCV) is a technique that uses microelectrodes (diameter ~5 µm, length ~150 µm) to monitor DA signaling in the striatum. FSCV studies with anesthetized rodents commonly use urethane or isoflurane anesthesia; however, the possible influence of isoflurane anesthesia under control conditions (i.e., saline treatment) on DA signaling has not been investigated. Male Sprague-Dawley rats (Rattus norvegicus) 300-500 grams were anesthetized with urethane or isoflurane anesthesia. Once fully anesthetized, rats were secured in a stereotaxic apparatus where their skin and fascia were removed to allow for the drilling of small holes (1-2 mm in diameter) for electrode placement. The reference electrode, coated with Ag/AgCl, was placed just below dura, the FSCV electrode was placed in the dorsal striatum (+1.2 AP, +2.0 ML, -5.0 DV), and the stimulating electrode was placed above the medial forebrain bundle (-4.6 AP, +1.4 ML, -7.5 DV). Biphasic pulses (60 Hz, 60 pulses, 300 µA) were sent through the bipolar stimulating electrode to evoke DA release. Once consistent DA signals were observed, DA signals were stimulated and recorded every 5 min for 1 hour after an intraperitoneal injection of saline. Preliminary data suggest an attenuation of DA signals under isoflurane anesthesia. Conversely, under urethane anesthesia, when temperature was carefully monitored and kept stable, DA signals remain relatively stable. Future directions involve investigating the influence of nicotine on DA signaling in the dorsal striatum.
Recommended Citation
Rosenbaum, Gracie E.; Ginder, Darren E.; Gainer, Mitchell P.; and Daberkow, David P., "The Influence of Anesthesia on Dopamine Signaling in the Rat Dorsal Striatum" (2024). 2024 Symposium. 21.
https://dc.ewu.edu/srcw_2024/ps_2024/p2_2024/21
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
The Influence of Anesthesia on Dopamine Signaling in the Rat Dorsal Striatum
PUB NCR
The striatum is a brain structure involved in reward learning and motor control. It receives input from the neurotransmitter dopamine (DA). Fast-scan cyclic voltammetry (FSCV) is a technique that uses microelectrodes (diameter ~5 µm, length ~150 µm) to monitor DA signaling in the striatum. FSCV studies with anesthetized rodents commonly use urethane or isoflurane anesthesia; however, the possible influence of isoflurane anesthesia under control conditions (i.e., saline treatment) on DA signaling has not been investigated. Male Sprague-Dawley rats (Rattus norvegicus) 300-500 grams were anesthetized with urethane or isoflurane anesthesia. Once fully anesthetized, rats were secured in a stereotaxic apparatus where their skin and fascia were removed to allow for the drilling of small holes (1-2 mm in diameter) for electrode placement. The reference electrode, coated with Ag/AgCl, was placed just below dura, the FSCV electrode was placed in the dorsal striatum (+1.2 AP, +2.0 ML, -5.0 DV), and the stimulating electrode was placed above the medial forebrain bundle (-4.6 AP, +1.4 ML, -7.5 DV). Biphasic pulses (60 Hz, 60 pulses, 300 µA) were sent through the bipolar stimulating electrode to evoke DA release. Once consistent DA signals were observed, DA signals were stimulated and recorded every 5 min for 1 hour after an intraperitoneal injection of saline. Preliminary data suggest an attenuation of DA signals under isoflurane anesthesia. Conversely, under urethane anesthesia, when temperature was carefully monitored and kept stable, DA signals remain relatively stable. Future directions involve investigating the influence of nicotine on DA signaling in the dorsal striatum.
