The Role of Exogenous Gibberellic Acid (GA3) Application in Breaking Seed Dormancy in Clematis hirsutissima

Faculty Mentor

Robin O'Quinn

Presentation Type

Poster

Start Date

5-8-2024 11:15 AM

End Date

5-8-2024 1:00 PM

Location

PUB NCR

Primary Discipline of Presentation

Biology

Abstract

Seed germination in plants is controlled by several mechanisms, including the presence of phytohormones like gibberellins, as well as environmental conditions, such as temperature, light, and moisture. Germination of dormant seeds can be promoted by the exogenous application of Gibberellic Acid (GA3). GA3 acts as a natural endogenous regulator of dormancy. Clematis hirsutissima, commonly known as Sugarbowls, in the plant family Ranunculaceae, is an important perennial species that contributes to biodiversity in western prairie ecosystems by providing habitat for pollinators and wildlife. This native to Washington, has been used for its medicinal properties by Indigenous peoples for thousands of years, and it is also used in contemporary horticulture for its distinct beauty. Here we report on the effect of exogenous GA3 application on germination in C. hirsutissima seeds across a cold stratification time gradient. We subjected GA3 treated and untreated (control) C. hirsutissima seeds to a cold moist stratification (4° C) for 14, 28, 56, and 84 days. Our hypotheses are that the application of GA3 will 1) decrease the length of cold stratification by helping to break dormancy earlier, and 2) increase the rate of germination. Treatment group averages will be compared using analysis of variance (ANOVA).

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May 8th, 11:15 AM May 8th, 1:00 PM

The Role of Exogenous Gibberellic Acid (GA3) Application in Breaking Seed Dormancy in Clematis hirsutissima

PUB NCR

Seed germination in plants is controlled by several mechanisms, including the presence of phytohormones like gibberellins, as well as environmental conditions, such as temperature, light, and moisture. Germination of dormant seeds can be promoted by the exogenous application of Gibberellic Acid (GA3). GA3 acts as a natural endogenous regulator of dormancy. Clematis hirsutissima, commonly known as Sugarbowls, in the plant family Ranunculaceae, is an important perennial species that contributes to biodiversity in western prairie ecosystems by providing habitat for pollinators and wildlife. This native to Washington, has been used for its medicinal properties by Indigenous peoples for thousands of years, and it is also used in contemporary horticulture for its distinct beauty. Here we report on the effect of exogenous GA3 application on germination in C. hirsutissima seeds across a cold stratification time gradient. We subjected GA3 treated and untreated (control) C. hirsutissima seeds to a cold moist stratification (4° C) for 14, 28, 56, and 84 days. Our hypotheses are that the application of GA3 will 1) decrease the length of cold stratification by helping to break dormancy earlier, and 2) increase the rate of germination. Treatment group averages will be compared using analysis of variance (ANOVA).