A Soil Analysis of Bacterial Antibiotic Producers

Faculty Mentor

Suzanne Bassett

Presentation Type

Poster

Start Date

5-8-2024 11:15 AM

End Date

5-8-2024 1:00 PM

Location

PUB NCR

Primary Discipline of Presentation

Biology

Abstract

Antibiotics are a very important medical resource as are the bacteria from which they are derived. While antibiotics have treated various infections and have saved many lives, over the years, the widespread use of antibiotics has led to its decrease in production. Additionally, the overuse of antibiotics has resulted in numerous resistant strains causing a serious threat to public health. Given the importance of antibiotics in treating diseases and the subsequent development of antibiotic resistance, a more diverse selection of antibiotics is required. For this reason, we are participating in the “Tiny Earth Research Project”. During our research we tested 47 bacterial isolates that were screened for antibiotic production. The Gram-positive tester strains were Staphylococcus epidermidis and Staphylococcus aureus, and the Gram-negative tester strain was Escherichia coli. A method of turtle tracks was used to screen for the most promising antibiotic producers among the original isolates. The identification of the top isolates was performed using the 16S rRNA sequencing of polymerase chain reaction products.

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May 8th, 11:15 AM May 8th, 1:00 PM

A Soil Analysis of Bacterial Antibiotic Producers

PUB NCR

Antibiotics are a very important medical resource as are the bacteria from which they are derived. While antibiotics have treated various infections and have saved many lives, over the years, the widespread use of antibiotics has led to its decrease in production. Additionally, the overuse of antibiotics has resulted in numerous resistant strains causing a serious threat to public health. Given the importance of antibiotics in treating diseases and the subsequent development of antibiotic resistance, a more diverse selection of antibiotics is required. For this reason, we are participating in the “Tiny Earth Research Project”. During our research we tested 47 bacterial isolates that were screened for antibiotic production. The Gram-positive tester strains were Staphylococcus epidermidis and Staphylococcus aureus, and the Gram-negative tester strain was Escherichia coli. A method of turtle tracks was used to screen for the most promising antibiotic producers among the original isolates. The identification of the top isolates was performed using the 16S rRNA sequencing of polymerase chain reaction products.