Analysis of Antibiotic Producers from Spokane Soil Samples

Faculty Mentor

Dr. Suzanne Bassett

Presentation Type

Poster

Start Date

5-7-2025 9:00 AM

End Date

5-7-2025 11:00 AM

Location

PUB NCR

Primary Discipline of Presentation

Biology

Abstract

SCC Research Club "EWU 2025 Poster" Abstract

Antibiotics and the bacteria from which they are derived are some of our most important and powerful medical resources. While antimicrobial/antibiotic-resistant strains of pathogens are growing in number, attributed to widespread and inappropriate use of antibiotics, investments in both research and production are declining worldwide. Lynn L Silver has coined the term ‘Discovery Void’ to describe the lack of innovation in the last half-century. Given the serious threat to public health, the importance of antibiotics to treat diseases, and the subsequent inevitability of novel strain resistance demanding a more diverse selection of antibiotics - we are participating in the “Tiny Earth Research Project.” As part of our research, we collected 15 soil samples and tested 24 bacterial isolates for antibiotic production. A method of turtle tracks was used to screen for the most promising antibiotic producers. The Gram-positive tester strains were Staphylococcus epidermidis and Staphylococcus aureus, and the Gram-negative tester strain was Escherichia coli. We identified the best performing isolates using the 16S rRNA sequencing of polymerase chain reaction products.

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SCC Research Club 2025 Poster for EWU

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May 7th, 9:00 AM May 7th, 11:00 AM

Analysis of Antibiotic Producers from Spokane Soil Samples

PUB NCR

SCC Research Club "EWU 2025 Poster" Abstract

Antibiotics and the bacteria from which they are derived are some of our most important and powerful medical resources. While antimicrobial/antibiotic-resistant strains of pathogens are growing in number, attributed to widespread and inappropriate use of antibiotics, investments in both research and production are declining worldwide. Lynn L Silver has coined the term ‘Discovery Void’ to describe the lack of innovation in the last half-century. Given the serious threat to public health, the importance of antibiotics to treat diseases, and the subsequent inevitability of novel strain resistance demanding a more diverse selection of antibiotics - we are participating in the “Tiny Earth Research Project.” As part of our research, we collected 15 soil samples and tested 24 bacterial isolates for antibiotic production. A method of turtle tracks was used to screen for the most promising antibiotic producers. The Gram-positive tester strains were Staphylococcus epidermidis and Staphylococcus aureus, and the Gram-negative tester strain was Escherichia coli. We identified the best performing isolates using the 16S rRNA sequencing of polymerase chain reaction products.