Investigating the influence of isoflurane and urethane anesthesia on dopamine signaling
Faculty Mentor
David Daberkow
Document Type
Oral Presentation
Start Date
10-5-2023 10:20 AM
End Date
10-5-2023 10:40 AM
Location
PUB 319
Department
Biology
Abstract
Dopamine (DA) is a neurotransmitter that innervates the striatum of the brain and is involved in reward learning and movement control. Fast-scan cyclic voltammetry (FSCV) is a technique that uses microelectrodes to monitor DA signaling in the brain. FSCV studies with anesthetized rodents usually use isoflurane or urethane anesthesia; however, the possible influence of these anesthetics on DA signaling has not been thoroughly investigated. Male Sprague-Dawley rats were anesthetized with urethane or isoflurane anesthesia. Once fully anesthetized, rats were secured in a stereotaxic apparatus where their skin and fascia were removed to allow for the drilling of small holes where electrodes were placed. The reference electrode, coated with Ag/AgCl, was placed just below dura, the FSCV electrode was placed in the striatum, and the stimulating electrode was placed above the medial forebrain bundle. Biphasic pulses (60 Hz, 60 pulses, 300 µA) were sent through the stimulating electrode to allow for the release of DA from neurons. Once consistent DA signals were observed, DA signals were stimulated and recorded for 1 hour. Preliminary data suggest an attenuation of the DA signals under isoflurane anesthesia when rat body temperature was not kept stable. Conversely, under urethane anesthesia, when temperature was carefully monitored and kept stable, DA signals remained relatively stable. Future directions involve carefully monitoring and keeping rat temperature stable under isoflurane anesthesia while monitoring DA with FSCV.
Recommended Citation
Rosenbaum, Gracie, "Investigating the influence of isoflurane and urethane anesthesia on dopamine signaling" (2023). 2023 Symposium. 3.
https://dc.ewu.edu/srcw_2023/res_2023/os2_2023/3
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Investigating the influence of isoflurane and urethane anesthesia on dopamine signaling
PUB 319
Dopamine (DA) is a neurotransmitter that innervates the striatum of the brain and is involved in reward learning and movement control. Fast-scan cyclic voltammetry (FSCV) is a technique that uses microelectrodes to monitor DA signaling in the brain. FSCV studies with anesthetized rodents usually use isoflurane or urethane anesthesia; however, the possible influence of these anesthetics on DA signaling has not been thoroughly investigated. Male Sprague-Dawley rats were anesthetized with urethane or isoflurane anesthesia. Once fully anesthetized, rats were secured in a stereotaxic apparatus where their skin and fascia were removed to allow for the drilling of small holes where electrodes were placed. The reference electrode, coated with Ag/AgCl, was placed just below dura, the FSCV electrode was placed in the striatum, and the stimulating electrode was placed above the medial forebrain bundle. Biphasic pulses (60 Hz, 60 pulses, 300 µA) were sent through the stimulating electrode to allow for the release of DA from neurons. Once consistent DA signals were observed, DA signals were stimulated and recorded for 1 hour. Preliminary data suggest an attenuation of the DA signals under isoflurane anesthesia when rat body temperature was not kept stable. Conversely, under urethane anesthesia, when temperature was carefully monitored and kept stable, DA signals remained relatively stable. Future directions involve carefully monitoring and keeping rat temperature stable under isoflurane anesthesia while monitoring DA with FSCV.
