Date of Award
Master of Science (MS) in Biology
"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance genes. These isolates were identified using the Vitek2 Identification System (BioMerieux, Durham N.C; U.S.A.). Sixty-seven percent of isolates were discovered to have plasmids and 54% had two or more plasmids. Plasmid size ranged from 2 to lOKb"--Document.
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Hartman, Gwendolyn Lenore, "An assessment of antibiotic resistant bacteria in biosolid used as garden fertilizer" (2012). EWU Masters Thesis Collection. 77.