Date of Award
2012
Rights
Access is available to all users
Document Type
Thesis
Degree Name
Master of Science (MS) in Biology
Department
Biology
Abstract
"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance genes. These isolates were identified using the Vitek2 Identification System (BioMerieux, Durham N.C; U.S.A.). Sixty-seven percent of isolates were discovered to have plasmids and 54% had two or more plasmids. Plasmid size ranged from 2 to lOKb"--Document.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Hartman, Gwendolyn Lenore, "An assessment of antibiotic resistant bacteria in biosolid used as garden fertilizer" (2012). EWU Masters Thesis Collection. 77.
https://dc.ewu.edu/theses/77
Comments
Typescript. Vita.