Faculty Mentor

Prakash Bhuta

Document Type

Article

Publication Date

2014

Department

Biology

Abstract

We developed a plasmid cloning by PCR technique for the green fluorescence protein gene. PCR was used to amplify the GFP gene on the pGLO plasmid with custom PCR primers. The primer design included three sequences: 1) a leader sequence, 2) a restriction endonuclease recognition sequence for the cloning site, and 3) a complimentary sequence to the GFP gene. The GFP gene amplicon was ligated to pre-cut pUC18. The recombinant pUC18 was transformed using chemically competent E. coli DH5a. A blue/white screening method was used to identify the recombinant clones.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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