Rhabdocline parkeri, an endophyte of Douglas fir, forms symptomless infections within single epidermal cells of healthy needles. Rhabdocline parkeri strains were isolated from trees growing in various habitats ranging from virgin old growth forests to a single young tree growing isolated in a meadow. Different genotypes of R. parkeri were distinguished by using the Random Amplified Polymorphic DNA (RAPD) technique. A significantly lower number of R. parkeri genotypes per unit foliage were isolated from trees within a 20-year-old managed stand and from an isolated tree than from old growth trees. Foliage from young trees (20 years old) in a stand directly adjacent to the sampled old growth trees showed levels of genotypic endophyte diversity comparable to that in the old growth trees. Genotypic diversity is estimated to be at least three orders of magnitude greater in foliage of the old growth trees and adjacent young trees than in foliage from the managed stand and from an isolated tree. This difference is ascribed to differences in tree age and access to inoculum.
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McCutcheon, Timothy L.; Carroll, George C.; and Schwab, Suzanne, "Genotypic Diversity In Populations Of A Fungal Endophyte From Douglas-Fir" (1993). Biology Faculty Publications. Paper 5.