Investigation of CSF1R Sialylation
Faculty Mentor
Dr. Jason Ashley
Presentation Type
Poster
Start Date
May 2025
End Date
May 2025
Location
PUB NCR
Primary Discipline of Presentation
Biology
Abstract
Colony-stimulating factor 1 receptor (CSF1R) plays a critical role in the survival and proliferation of myeloid lineage cells, including specialized immune cells such as macrophages. Polysialic acid, a carbohydrate polymer, is thought to be involved in various biological processes, including tumor growth, synaptic plasticity, and the immune system pertaining to cell-to-cell signaling and interactions. Previous research suggests that CSF1R may be decorated with polysialic acid. In this current study, molecular techniques, including immunoprecipitation to isolate CSF1R and western blotting with polysialic acid-specific antibodies, were employed to investigate this possibility. While definitive evidence for polysialylation of CSF1R cannot yet be confirmed, initial findings showed banding in whole cell lysates with polysialic acid-detecting antibodies, indicating that polysialylation occurs in macrophages. Although it remains unclear whether CSF1R is directly polysialylated, these results suggest a broader occurrence of polysialylation in macrophages. Further investigation is required to explain the full extent of polysialic acid’s involvement in macrophage biology. If these findings are confirmed, they could open new avenues for understanding the role of polysialic acid in immune regulation and macrophages.
Recommended Citation
Larson, Hannah and Ashley, Jason PhD., "Investigation of CSF1R Sialylation" (2025). 2025 Symposium. 12.
https://dc.ewu.edu/srcw_2025/ps_2025/p2_2025/12
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Investigation of CSF1R Sialylation
PUB NCR
Colony-stimulating factor 1 receptor (CSF1R) plays a critical role in the survival and proliferation of myeloid lineage cells, including specialized immune cells such as macrophages. Polysialic acid, a carbohydrate polymer, is thought to be involved in various biological processes, including tumor growth, synaptic plasticity, and the immune system pertaining to cell-to-cell signaling and interactions. Previous research suggests that CSF1R may be decorated with polysialic acid. In this current study, molecular techniques, including immunoprecipitation to isolate CSF1R and western blotting with polysialic acid-specific antibodies, were employed to investigate this possibility. While definitive evidence for polysialylation of CSF1R cannot yet be confirmed, initial findings showed banding in whole cell lysates with polysialic acid-detecting antibodies, indicating that polysialylation occurs in macrophages. Although it remains unclear whether CSF1R is directly polysialylated, these results suggest a broader occurrence of polysialylation in macrophages. Further investigation is required to explain the full extent of polysialic acid’s involvement in macrophage biology. If these findings are confirmed, they could open new avenues for understanding the role of polysialic acid in immune regulation and macrophages.
