Date of Award

2013

Document Type

Thesis

Degree Name

Master of Science (MS) in Biology

Department

Biology

First Advisor

Andrea Castillo

Second Advisor

Prakash Bhuta

Third Advisor

Nicholas Burgis

Abstract

"Helicobacter pylori is a spiral, gram-negative bacterium that colonizes the stomachs of approximately 50% of the World's population overall and is a major etiological agent of human gastric adenocarcinoma. Of infected individuals, only 10-15% develop severe gastric disease due to environmental factors, host genetic factors, and more significantly, genetic differences in the infecting H pylori strains. Type I strains of H pylori contain a 40-kb cytotoxin-associated pathogenicity island (cag PAl) that encodes and secretes the CagA protein into host epithelial cells via a type IV secretion system. To date, CagA is the only identified effector protein of the cag PAI. The goal of this study was to identify novel H pylori virulence factors, to further elucidate their role in H pylori virulence and their potential as novel effectors of the cag PAI. In the work presented here, we generated an H pylori genomic plasmid library and screened this library in Saccharomyces cerevisiae for toxic effects. We initially identified 2 candidate H pylori virulence factors, however, after further analysis these candidates were not toxic to S. cerevisiae and are no longer genes of interest. To identify novel H pylori virulence factors, others in the lab are addressing pitfalls found in this study to conduct a better-structured screen that we believe will be successful in identifying H pylori genes of interest"--Document.

Comments

Typescript. Vita.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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